Multidrug resistance in patients undergoing resective epilepsy surgery is not associated with C3435T polymorphism in the ABCB1 (MDR1) gene

Dericioglu, Nese; Babaoglu, Melih O.; Yasar, Umit; Bal, I. Burak; Bozkurt, Atila; Saygi, Serap

doi:10.1016/j.eplepsyres.2008.03.004

« Previous Next »Epilepsy Research
Volume 80, Issue 1 , Pages 42-46, July 2008

Multidrug resistance in patients undergoing resective epilepsy surgery is not associated with C3435T polymorphism in the ABCB1 (MDR1) gene

Nese Dericioglu
- Hacettepe University, Institute of Neurological Sciences and Psychiatry, Sihhiye 06100, Ankara, Turkey
- Corresponding author. Fax: +90 312 309 3451.
Melih O. Babaoglu
- Hacettepe University, School of Medicine, Department of Pharmacology, Sihhiye 06100, Ankara, Turkey
- Fax: +90 312 310 5312.
Umit Yasar
- Hacettepe University, School of Medicine, Department of Pharmacology, Sihhiye 06100, Ankara, Turkey
- Fax: +90 312 310 5312.
I. Burak Bal
- Hacettepe University, School of Medicine, Department of Pharmacology, Sihhiye 06100, Ankara, Turkey
- Fax: +90 312 310 5312.
Atila Bozkurt
- Hacettepe University, School of Medicine, Department of Pharmacology, Sihhiye 06100, Ankara, Turkey
- Fax: +90 312 310 5312.
Serap Saygi
- Hacettepe University, School of Medicine, Department of Neurology, Sihhiye 06100, Ankara, Turkey
- Fax: +90 312 309 3451.

Received 30 July 2007; received in revised form 25 January 2008; accepted 5 March 2008.

Summary

Purpose

The C3435T polymorphism in the gene coding for P-glycoprotein (ABCB1) has been correlated with drug resistance in patients with epilepsy. However, replication studies have revealed conflicting results and the reason for this is not clear. We investigated the frequency of C3435T polymorphism in epileptic Turkish patients who underwent resective epilepsy surgery and compared our results with healthy controls.

Methods

DNA samples were obtained from 100 healthy controls and 89 consecutive adult patients who underwent resective brain surgery due to refractory seizures at our epilepsy center. Genotypes for the C3435T polymorphism were determined by PCR and restriction analysis.

Results

Comparison of drug-resistant patients and healthy controls revealed no significant difference in allele frequency (C vs. T; χ²=0.015, p=0.90) and genotype frequency (χ²=2.05, p=0.36). The findings in the pure hippocampal sclerosis (HS) group (n=73) were not significantly different from control subjects, either (allele frequency: χ²=0.29, p=0.59; genotype frequency: χ²=2.14, p=0.34).

Conclusions

Our findings failed to prove an association between C3435T polymorphism and drug resistance in a sample of Turkish patients with refractory epilepsy who underwent resective brain surgery.

Keywords: Multidrug resistance, Refractory epilepsy, Epilepsy surgery, ABCB1 (MDR1) gene, C3435T polymorphism