Epilepsy Research
Volume 22, Issue 1 , Pages 23-34 , September 1995

Epileptic discharges induced by pentylenetetrazol: Ultrastructural alterations in identified neurons and glial cells (Helix pomatia)

  • Andreas Schulze-Bonhage

      Affiliations

    • Institut für Experimentelle Epilepsieforschung der Universität Münster, Hüfferstr. 68, 48149 Münster, Germany
    • ,
  • Martin Wiemann

      Affiliations

    • Institut für Experimentelle Epilepsieforschung der Universität Münster, Hüfferstr. 68, 48149 Münster, Germany
    • ,
  • Ulrich Altrup

      Affiliations

    • Corresponding Author InformationCorresponding author.
    • Institut für Experimentelle Epilepsieforschung der Universität Münster, Hüfferstr. 68, 48149 Münster, Germany
    • ,
  • Werner Wittkowski

      Affiliations

    • Institut für Anatomie der Universität Münster, Vesaliusweg 2-4, 48149 Münster, Germany
    • ,
  • Erwin-Josef Speckmann

      Affiliations

    • Institut für Physiologie, Robert-Koch-Str. 27a, and Institut für Experimentelle Epilepsieforschung der Universität Münster, Hüfferstr. 68, 48149 Münster, Germany

Received 29 November 1994 ,Accepted 11 May 1995.

References 

  1. Altrup U. Inputs and outputs of giant neurons B1 and B2 in the buccal ganglia of Helix pomatia: An electrophysiological and morphological study. Brain Res. 1987;414:271–284
  2. Altrup U, Lehmenkühler A, Madeja M, Speckmann E-J. Morphology and function of the identified neuron B3 in the buccal ganglia of Helix pomatia. Comp. Biochem. Physiol. 1990;97A:65–74
  3. Altrup U, Lehmenkühler A, Speckmann E-J. Effects of the hypnotic drug etomidate in a model nervous system (buccal ganglia, Helix pomatia). Comp. Biochem. Physiol. 1991;99C:579–587
  4. Altrup U, Speckmann E-J. Intrasomatically recorded action potentials in snail neurons (Helix pomatia): different shapes with different sites of origin in the neuronal arborization. A combined morphological and electrophysiological study. Comp. Biochem. Physiol. 1984;77A:225–230
  5. Altrup U, Speckmann E-J. Epileptic discharges induced by pentylenetetrazol: Changes of shape of dendrites. Brain Res. 1988;456:401–405
  6. Babb TL, Kupfer WR, Pretorius JK, Crandall PH, Levesque MF. Synaptic reorganization by mossy fibers in human epileptic fascia dentata. Neuroscience. 1991;42:351–363
  7. Bertram EH, Lothman WW, Lenn NJ. The hippocampus in experimental chronic epilepsy: A morphometric analysis. Ann. Neurol. 1990;27:43–48
  8. Brown WJ. Structural substrates of seizure foci in the human temporal lobe. (A combined electrophysiological optical microscopic and ultrastructural study.). In:  Brazier MAB editors. Epilepsy: Its Phenomena in Man. New York: Academic Press; 1973;p. 339–374
  9. Brown WJ, Babb TL. Effects of repeated seizures on hippocampal neurons in the cat. In: 2nd ed..  Delgado-Escueta AV,  Wasterlain CG,  Treiman DM,  Porter RJ editor. Advances in Neurology, Vol. 34: Status Epilepticus. New York: Raven Press; 1983;p. 161–168
  10. Chalazonitis N, Takeuchi H. Amples oscillations du potential de membrane induites par le metrazol (neurones autoactifs d'Helix pomatia). C.R. Soc. Biol. (Paris). 1968;162:1552–1554
  11. Collins RC, Lothman EW, Olney JW. Status epilepticus in the limbic system: Biochemical and pathological changes. In:  Delgado-Escueta AV,  Wasterlain CG,  Treiman DM,  Porter RJ editor. Status Epilepticus. New York: Raven Press; 1983;p. 277–288
  12. Evans M, Griffiths T, Meldrum B. Early changes in the rat hippocampus following seizures induced by bicuculline or 1-allylglycine: A light and electron microscope study. Neuropathol. Appl. Neurobiol. 1983;9:39–52
  13. DeGiorgio CM, Tomiyasu U, Gott PS, Treiman DM. Hippocampal pyramidal cell loss in human status epilepticus. Epilepsia. 1992;33:23–27
  14. Holmes GL. Do seizures cause brain damage?. Epilepsia. 1991;32(Suppl. 5):S14–S28
  15. Kandel ER. Cellular Basis of Behavior: An Introduction to Behavioral Neurobiology. San Francisco: Freeman; 1976;
  16. Lücke A, Speckmann E-J, Altrup U, Lehmenkühler A, Walden J. Decrease of free calcium concentration at the outer surface of identified snail neurons during paroxysmal depolarization shifts. Neurosci. Lett. 1990;112:190–193
  17. Madeja M, Altrup U, Speckmann E-J. Synchronization of epileptic discharges: Temporal coupling of paroxysmal depolarizations in the buccal ganglia of Helix pomatia. Comp. Biochem. Physiol. 1989;94C:585–590
  18. Meldrum B. Excitotoxicity and epileptic brain damage. Epilepsy Res. 1991;10:55–61
  19. Meldrum BS. Cell damage in epilepsy and the role of calcium in cytotoxicity. Adv. Neurol. 1986;44:849–855
  20. Morgan JI, Curran T. Proto-oncogene transciption factors and epilepsy. Trends Pharmacol. Sci. 1991;12:343–349
  21. Nolte A, Breucker H, Kuhlmann D. Cytosomale Einschl̈usse und Neurosekret im Nervengewebe von Gastropoden. Z. Zellforsch. 1965;68:1–27
  22. Olney JW, DeGubareff T, Sloviter RS. ‘Epileptic’ brain damage in rats induced by sustained electrical stimulation of the perforant path. II. Ultrastructural analysis of acute hippocampal pathology. Brain Res. Bull. 1983;10:699–712
  23. Phelps S, Mitchell J, Wheal HV. Changes to synpatic ultrastructure in field CA1 of the rat hippocampus following intracerebroventricular injection of kainic acid. Neuroscience. 1991;40:687–699
  24. Reinecke M. Die Gliazellen der Cerebralganglien von Helix pomatia L. (Gastropoda: Pulmonata). I. Ultrastruktur and Organsation. Zoomorphologie. 1975;82:105–136
  25. Ribak CE. Contemporary methods in neurocytology and their application to the study of epilepsy. In: 2nd ed..  DelgadoEscueta AV,  Ward AA,  Woodbury DM,  Porter RJ editor. Advances in Neurology. Vol. 44:New York: Raven Press; 1986;p. 739–764
  26. Scheibel AB. Morphological correlates of epilepsy: Cells in the hippocampus. In:  Glaser GH,  Penry JK,  Woodbury DM editor. Antiepileptic Drugs: Mechanisms of Action. New York: Raven Press; 1980;p. 49–60
  27. Schormair C, Bingmann D, Wittkowski W, Speckmann E-J. Morphology of CA3 neurons in hippocampal slices with nonepileptic and epileptic activity: a light and electron microscopic study. Brain Res. Bull. 1993;32:329–338
  28. Schulze H, Speckmann E-J, Kuhlmann D, Caspers H. Topography and bioelectrical properties of identifiable neurons in the buccal ganglion of Helix pomatia. Neurosci. Lett. 1975;1:277–281
  29. Schulze-Bonhage A, Altrup U, Speckmann EA, Wittkowski W. Photodamage induced by confocal laser scanning investigation of Lucifer Yellow-stained neurons in the buccal ganglia of Helix pomatia. In:  Elsner N,  Richter D editor. Rhythmogenesis in Neurons and Networks: Proceedings of the 20th Göttingen Neurobiology Conference. Stuttgart: Georg Thieme Verlag; 1992;p. 744
  30. Schulze-Bonhage A, Altrup U, Speckmann E-J, Wittkowski W. Structure and bioelectricity of single neurons of Helix pomatia in the intact nervous tissue during epileptic activity: Simultaneous evaluations by confocal microscopy and intracellular recordings of membrane potential changes. Comp. Biochem. Physiol. 1993;106A:537–545
  31. Sloviter RS. ‘Epileptic’ brain damage in rats induced by sustained electrical stimulation of the perforant path. I. Acute electrophysiological and light microscopic studies. Brain Res. Bull. 1983;10:675–697
  32. Speckmann E-J, Caspers H. Paroxysmal depolarization and changes in action potentials induced by pentylenetetrazol in isolated neurons of Helix pomatia. Epilepsia. 1973;14:397–408
  33. Steffens H. The buccal ganglia of Helix pomatia L. (Gastropoda, Pulmonata). Zoomorphologie. 1980;95:195–212
  34. Sugaya E, Onozuka M. Intracellular calcium: Its release from granules during bursting activity in snail neurons. Science. 1978;202:1195–1197

PII: 0920-1211(95)00031-5

Epilepsy Research
Volume 22, Issue 1 , Pages 23-34 , September 1995

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